Dr Bruno Oliveira
FCT Stimulus Assistant Researcher
E-mail: bruno.oliveira@medicina.ulisboa.pt

I joined the GBernardes group in Lisbon as a FCT Stimulus Assistant Researcher in January 2019 to investigate new cleavable reactions for prodrug activation by using platinum-based chemotherapeutics. Before then, I had joined the GBernardes group in Cambridge in 2015 as a Marie-Curie fellow to work on the development of new bioorthogonal methods for site-selective protein modification and/or drug release exploring the inverse electron demand Diels–Alder reaction.

I obtained my PhD (2012) and Masters degree (2007) in Nuclear Medicine synthesizing new chemical probes for molecular imaging of the Nitric Oxide Synthase enzyme (PET, SPECT). During my NIH Postdoctoral Fellowship at the Massachusetts General Hospital/Harvard Medical School (2013–2015), my work at the Peter Caravan Lab focused on the discovery of PET probes based on fibrin-binding peptides for thrombus imaging.

Areas of expertise:
Protein modification, chemical-triggered drug activation, bioorthogonal/click chemistry, bioinorganic chemistry and bioorganometallic chemistry



17. In vivo pretargeting based on cysteine-selective antibody modification with IEDDA bioorthogonal handles for click chemistry
Ferreira VFC; Oliveira BL*; D’Onofrio A; Farinha CM; Gano L; Paulo A; Bernardes GJL*; Mendes F* (co-senior authors)
Bioconjugate Chem. 2021, 32, 121–132

16. Platinum-triggered bond-cleavage of pentynoyl amide and N-propargyl handles for drug-activation
Oliveira BL; Stenton BJ; Unnikrishnan VB; de Almeida CR; Conde J; Negrão M; Schneider FSS; Cordeiro C; Ferreira MG; Caramori GF; Domingos JB; Fior R; Bernardes GJL*
J. Am. Chem. Soc. 2020, 142, 10869–10880

15. Mechanistic insights into transition metal-mediated bioorthogonal uncaging reactions
Latocheski E; Dal Forno GM; Ferreira TM; Oliveira BL; Bernardes GJL; Domingos JB
Chem. Soc. Rev. 2020, 49, 7710–7729

14. Development of a self-immolative linker for tetrazine-triggered release of alcohols in cells
Davies S; Oliveira BL; Bernardes GJL*
Org. Biomol. Chem. 2019, 17, 5727–5730

13. Azabicyclic vinyl sulfones for residue-specific dual protein labelling
Gil de Montes E; Jiménez-Moreno E; Oliveira BL; Navo CD; Cal PMSD; Jiménez-Osés G; Robina I; Moreno-Vargas AJ; Bernardes GJL*
Chem. Sci. 2019, 10, 4515–4522

12. Tetrazine-triggered release of carboxylic-acid-containing molecules for activation of an anti-inflammatory drug
Davies S; Qiao J; Oliveira BL; Navoc CD; Jiménez-Osésc G; Bernardes GJL*
ChemBioChem. 2019, 20, 1541–1546

11. Norbornene probes for the detection of cysteine sulfenic acid in cells
Alcock LJ; Oliveira BL; Deery MJ; Pukala TL; Perkins MV; Bernardes GJL*; Chalker JM
ACS Chem. Biol. 2019, 14, 594−598

10. Contemporary approaches to site-selective protein modification
Hoyt EA; Cal PMSD; Oliveira BL; Bernardes GJL*
Nat. Rev. Chem. 2019,3, 147–171

9. A fluorogenic probe for cell surface phosphatidylserine using an intramolecular indicator displacement sensing mechanism
Zwicker V; Oliveira BL; Yeo JH; Fraser ST; Bernardes GJL; New EJ; Jolliffe KA
Angew. Chem. Int. Ed. 2019, 58, 3087–3091

8. Radical-mediated thiol-ene strategy for photoactivation of thiol-containing drugs in cancer cells
Sun S; Oliveira BL; Jiménez-Osés G; Bernardes GJL*
Angew. Chem. Int. Ed. 2018, 57, 15832–15835

7. A thioether-directed palladium-cleavable linker for targeted bioorthogonal drug decaging
Stenton BJ; Oliveira BL; Matos MJ; Sinatra L; Bernardes GJL*
Chem. Sci. 2018, 9, 4185–4189

6Chemo and regioselective lysine modification on native proteins
Matos MJ; Oliveira BL; Martínez-Sáez N; Guerreiro A; Cal PMSD; Bertoldo J; Maneiro M; Perkins E; Howard J; Deery MJ; Chalker JM; Corzana F; Jiménez-Osés G; Bernardes GJL*
J. Am. Chem. Soc. 2018140, 4004–4017

Highlighted in Chem. Eng. News 2018, 96, 13 “Computer-designed reagent targets lysine for protein modification”; Phys.orgHow to spark a chemical chain reaction”, March 9, 2018

5. Inverse electron demand Diels–Alder reactions in chemical biology
Oliveira BL; Guo Z; Bernardes GJL*
Chem. Soc. Rev2017, 46, 4895–4950

4. Vinyl ether–tetrazine pair for the traceless release of alcohols in cells
Jiménez-Moreno E; Guo Z; Oliveira BL; Albuquerque IS; Kitowski A; Guerreiro A; Boutureira O; Rodrigues T; Jiménez-Osés G; Bernardes GJL*
Angew. Chem. Int. Ed. 2017, 56, 243–247

3. A minimal, unstrained S-allyl handle for pre-targeting Diels–Alder bioorthogonal labelling in live cells
Oliveira BL, Guo Z; Boutureira O; Guerreiro A; Jiménez-Osés G; Bernardes GJL*
Angew. Chem. Int. Ed. 2016, 55, 14683–14687

2. Stoichiometric and irreversible cysteine-selective protein modification using carbonylacrylic reagents
Bernardim B; Cal PMSD; Matos MJ; Oliveira BL; Martínez-Sáez N; Albuquerque IS; Corzana F; Burtoloso ACB; Jiménez-Osés G; Bernardes GJL*
Nat. Commun. 2016, 7, 13128

1. Collagen labelling with an azide-proline chemical reporter in live cells
Amgarten B; Rajan R; Martínez-Sáez N; Oliveira BL; Albuquerque IS; Reid DG; Brooks RA; Duer MJ; Bernardes GJL*
Chem. Commun. 2015, 51, 5250–5252

Emerging Investigators Issue 2015